0488245 - ÚŽFG 2018 RIV US eng M - Část monografie knihy
Benešová, Veronika - Kinterová, Veronika - Kaňka, Jiří - Toralová, Tereza
Potential Involment of SCF - Complex in Zygotic Genome Activation During Early Bovine Embryo Development.
Zygotic Genome Activation. Vol. 1605. -: Springer, 2017 - (Kiho, L.), s. 245-257. ISBN 978-1-4939-6988-3
Grant CEP: GA ČR GP13-24730P
Institucionální podpora: RVO:67985904
Klíčová slova: SCF-complex * preimplantation development * qRT-PCR
Obor OECD: Developmental biology
DOI: https://doi.org/10.1007/978-1-4939-6988-3

Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
Trvalý link: http://hdl.handle.net/11104/0282842