Počet záznamů: 1  

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones

  1. 1.
    0488090 - ÚMG 2018 RIV US eng J - Článek v odborném periodiku
    Malinová, Anna - Cvačková, Zuzana - Matějů, Daniel - Hořejší, Zuzana - Abeza, C. - Vandermoere, F. - Bertrand, E. - Staněk, David - Verheggen, C.
    Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.
    Journal of Cell Biology. Roč. 216, č. 6 (2017), s. 1579-1596. ISSN 0021-9525. E-ISSN 1540-8140
    Grant CEP: GA ČR GPP301/12/P425; GA ČR GA15-00790S; GA ČR(CZ) GA14-34264S; GA MŠMT LO1419
    Institucionální podpora: RVO:68378050
    Klíčová slova: dominant retinitis-pigmentosa * splicing factor prp8 * rna-polymerase-ii * structural basis * spliceosomal snrnps * coiled bodies * cajal bodies * r2tp complex * mutations * biogenesis
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 8.784, rok: 2017

    Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFT UD2 and SNR NP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFT UD2, and SNR NP200 in association with the HSP90/R2TP complex, its ZNH IT2 cofactor, and additional proteins. HSP90 and R2TP bind unassembled U5 proteins in the cytoplasm, stabilize them, and promote the formation of the U5 snRNP. We further found that PRPF8 mutants causing Retinitis pigmentosa assemble less efficiently with the U5 snRNP and bind more strongly to R2TP, with one mutant retained in the cytoplasm in an R2TP-dependent manner. We propose that the HSP90/R2TP chaperone system promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8. The proteomics data further reveal new interactions between R2TP and the tuberous sclerosis complex (TSC), pointing to a potential link between growth signals and the assembly of key cellular machines.
    Trvalý link: http://hdl.handle.net/11104/0282716

     
    Název souboruStaženoVelikostKomentářVerzePřístup
    J_Cell_Biology_A_Malinova_2017.pdf23 MBVydavatelský postprintvyžádat
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.