Evaluation of strategies for humanization of the entire porcine HTT locus

0485164 - ÚŽFG 2018 CZ eng K - Konferenční příspěvek (tuzemská konf.)
Vochozková, Petra - Klymiuk, N. - Wolf, E. - Ellederová, Zdeňka - Motlík, Jan
Evaluation of strategies for humanization of the entire porcine HTT locus.
The 4th Animal Models of Neurodegenerative Diseases. Liběchov: ÚŽFG AV ČR, v. v. i., 2017, s. 25-26.
[Animal Models of Neurodegenerative Diseases /4./. Liblice (CZ), 22.10.2017-24.10.2017]
Grant CEP: GA MŠMT(CZ) LO1609; GA MŠMT 7F14308
Institucionální podpora: RVO:67985904
Klíčová slova: minipig model * HTT locus
Obor OECD: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Because fully suitable large animal models are still lacking for Huntington´s disease, we would like to generate a new minipig model which will have an entirely humanized HTT locus. Given the large size of the HTT gene (approx. 160 kb) we will test two different approaches to humanize the porcine HTT locus in porcine kidney cells (PKCs).
Trvalý link: http://hdl.handle.net/11104/0280237