Počet záznamů: 1  

Molecular survey of Rickettsial organisms in ectoparasites from a dog shelter in Northern Mexico

  1. 1.
    0483976 - BC 2018 RIV GB eng J - Článek v odborném periodiku
    González-Álvarez, V. H. - Fernández de Mera, I.G. - Cabezas Cruz, Alejandro - de la Fuente, J. - Ortega-Morales, A. I. - Almazán, C.
    Molecular survey of Rickettsial organisms in ectoparasites from a dog shelter in Northern Mexico.
    Veterinary Parasitology: Regional Studies and Reports. Roč. 10, December (2017), s. 143-148. E-ISSN 2405-9390
    Institucionální podpora: RVO:60077344
    Klíčová slova: Rhipicephalus sanguineus * Ctenocephalides felis * Heterodoxus spiniger * Rickettsia felis * Wolbachia pipientis * Dogs
    Obor OECD: Biochemistry and molecular biology

    The objective of this study was to screen and identify rickettsial organisms in ectoparasites collected from dogs in a shelter in Gomez Palacio, Durango, Mexico. One hundred dogs were inspected for ectoparasites. All the dogs were parasitized with Rhipicephalus sanguineus ticks, three with Heterodoxus spiniger lice and one with Ctenocephalides felis fleas. DNA was extracted from the ectoparasites found on each dog, and PCR with the primers for the Anaplasmataceae 16S rRNA and citrate synthase gltA genes were performed. Eight DNA samples obtained from ticks, three from lice and one from fleas were positive to 16S rRNA. Only one sample from C. felis and one from H. spiniger were positive to gltA. Sequence analysis of amplified products from C. felis showed identity to Rickettsia felis, Wolbachia pipientis, and Wolbachia spp., while a sequence from H. spiniger showed identity to Wolbachia spp. Herein we report the molecular detection of R. felis, W. pipientis, and Wolbachia spp. in C. felis and H. spiniger in northern Mexico. These results contribute to the knowledge of the microorganisms present in ectoparasites from dogs in Mexico.
    Trvalý link: http://hdl.handle.net/11104/0279149

     
     
Počet záznamů: 1  

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