Počet záznamů: 1  

Size-exclusion chromatography and its optimization for material science

  1. 1. 0481739 - UMCH-V 2018 RIV US eng J - Článek v odborném periodiku
    Netopilík, Miloš - Trhlíková, Olga
    Size-exclusion chromatography and its optimization for material science.
    Journal of Material Sciences & Engineering. Roč. 6, č. 4 (2017), s. 1-5, č. článku 358. ISSN 2169-0022
    Grant CEP: GA ČR(CZ) GC17-04258J
    Grant ostatní:OPPK(XE) CZ.2.16/3.1.00/24504; AV ČR(CZ) ASRT-16-02
    Program:Bilaterální spolupráce
    Institucionální podpora: RVO:61389013
    Klíčová slova: separation mechanism * size-exclusion chromatography * band broadening function
    Kód oboru RIV: CD - Makromolekulární chemie
    Obor OECD: Polymer science

    A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, judged by statistical properties of the curves, both skew and excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction. In analyses of polystyrene standards by size exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the longitudinal diffusion is not important even at low flow-rates.
    Trvalý link: http://hdl.handle.net/11104/0280730