Počet záznamů: 1  

Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)

  1. 1.
    0478519 - ÚŽFG 2018 RIV GB eng J - Článek v odborném periodiku
    Killer, Jiří - Pechar, R. - Švec, P. - Salmonová, H. - Švejstil, R. - Geigerová, M. - Rada, V. - Vlková, E. - Mekadim, Ch.
    Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus).
    International Journal of Systematic and Evolutionary Microbiology. Roč. 67, č. 7 (2017), s. 2903-2909. ISSN 1466-5026. E-ISSN 1466-5034
    Grant CEP: GA ČR GA13-08803S; GA MZe QJ1510338
    Institucionální podpora: RVO:67985904
    Klíčová slova: Lactobacillus sp. nov. * oral cavity * guinea pig
    Obor OECD: Microbiology
    Impakt faktor: 1.932, rok: 2017

    A Gram-stain-positive, facultatively anaerobic, and catalase-and oxidase-negative bacterial strain designated MOZM2(T), having 98.4% 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016(T), was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42% degree of genetic similarity between the novel strain and L. reuteri DSM 20016(T). Strain MOZM2(T) degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2(T) and L. reuteri DSM 20016(T) in their proportions of C-8:0, C-14:1, C-17:0, C-18:2 omega 6t and C-20:0 fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G +C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name Lactobacillus caviae sp. nov. is proposed, of which the type strain is MOZM2(T) (= CCM 8609(T) = DSM 100239(T) = LMG 28780(T)).
    Trvalý link: http://hdl.handle.net/11104/0274617

     
     
Počet záznamů: 1  

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