Počet záznamů: 1  

RGB color coded images in scanning electron microscopy of biological surfaces

  1. 1. 0478391 - MBU-M 2018 RIV US eng J - Článek v odborném periodiku
    Kofroňová, Olga - Benada, Oldřich
    RGB color coded images in scanning electron microscopy of biological surfaces.
    Acta virologica. Roč. 61, č. 3 (2017), s. 349-352 ISSN 0001-723X
    Grant CEP: GA MŠk(CZ) LO1509; GA ČR(CZ) GA16-20229S
    Institucionální podpora: RVO:61388971
    Klíčová slova: Biological surfaces * Color images * Scanning electron microscopy
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Obor OECD: Microbiology
    Impakt faktor: 0.696, rok: 2017

    We present here a methodological approach for the creation of color images in scanning electron microscopy by processing grayscale images taken simultaneously from at least three different detectors in a scanning electron microscope. The final color images are then produced by merging together those grayscale images in RGB color space. We show the images from non-conductive standard sample together with those obtained from real microbiological samples. The first one represents a microbial biofilm naturally grown on fiber glass filter. The other shows individual Bacillus subtilis cells from batch culture. All the image handling was done in open source image processing software ImageJ or GNU Image Manipulation Program (Gimp) or, alternatively, in proprietary AnalySis 3.2 Pro software processing suite.
    Trvalý link: http://hdl.handle.net/11104/0274508