Effect of yeasts on biodegradation potential of immobilized cultures of white rot fungi

0478001 - MBÚ 2018 RIV NL eng J - Článek v odborném periodiku
Šlosarčíková, P. - Novotný, Čeněk - Malachová, K. - Válková, H. - Fojtík, J.
Effect of yeasts on biodegradation potential of immobilized cultures of white rot fungi.
Science of the Total Environment. Roč. 589, JUL 1 (2017), s. 146-152. ISSN 0048-9697. E-ISSN 1879-1026
Institucionální podpora: RVO:61388971
Klíčová slova: Mixed culture * Fungal biofilm * Yeasts
Obor OECD: Microbiology
Impakt faktor: 4.610, rok: 2017

The aim was to investigate the effect of yeast organisms on the degradation process by immobilized cultures of ligninolytic fungi. Immobilization was accomplished by 7-day colonization of polyamide mesh with mycelial fragments. Irpex lacteus decolorized >90% of the initial concentration of 150 mg1(-1) of anthraquinone Remazol Brilliant Blue R dye in three subsequent decolorization cycles and the degradation capacity was not negatively affected by the presence of 10(6) Saccharomyces cerevisiae cells per ml in the mixed culture. The yeast was not able to degrade the dye. I. lacteus biofilm was also resistant to bacterial infection with E. coli. Inoculation of the yeast to pre-formed I. lacteus biofilm culture resulted in a reduction of fungal biomass by 27%. Levels of LiP, MnP and laccase of I. lacteus were not much influenced by S. cerevisiae or E. coli. Similar resilience of P. ostreatus biofilms was observed after exposure to yeast Issatchenkia occidentalis when the fungal degradation capacity measured with Reactive Orange 16 azo dye was maintained over two decolorization cycles. I. occidentalis did not degrade the dye under the conditions used. Formation of densely packed fungal biofilms with abundant extracellular polysaccharide was not impeded by the yeast. Increase of MnP and laccase levels attributable to the presence of A occidentalis was observed
Trvalý link: http://hdl.handle.net/11104/0274247