Počet záznamů: 1  

Environmentally sensitive probes for monitoring protein-membrane interactions at nanomolar concentrations

  1. 1.
    0475308 - UOCHB-X 2018 RIV NL eng J - Článek v odborném periodiku
    Shvadchak, Volodymyr V. - Kucherak, Oleksandr - Afitska, Kseniia - Dziuba, D. - Yushchenko, Dmytro A.
    Environmentally sensitive probes for monitoring protein-membrane interactions at nanomolar concentrations.
    Biochimica Et Biophysica Acta-Biomembranes. Roč. 1859, č. 5 (2017), s. 852-859. ISSN 0005-2736
    Institucionální podpora: RVO:61388963
    Klíčová slova: solvatochromic probes * fluorescence * protein-membrane interaction * affinity * binding stoichiometry * alpha-synuclein
    Kód oboru RIV: CE - Biochemie
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 3.438, rok: 2017

    Solvatochromic probes are suitable tools for quantitative characterization of protein-membrane interactions. Based on diverse fluorophores these probes have different fluorescent properties and therefore demonstrate different responses when applied for sensing the interactions of biomolecules. Surprisingly, to the best of our knowledge, no systematic comparison of the sensitivities of solvatochromic dyes for monitoring protein-membrane interactions was described. Hence, a rational choice of an optimal environmentally sensitive probe for such experiments is usually not a straightforward task. In this work we developed a series of thiol-reactive fluorescent probes based on the fluorophores with high sensitivity to their environment and compared them with two widely used DNS and DMN probes. We investigated the responses of these probes to the interaction of probe-labeled presynaptic protein alpha-synuclein with lipid membranes. We observed that newly synthesized probes based on fluorene and chromone dyes, which combine the strongest brightness and significant changes of fluorescence intensity, demonstrated the highest sensitivity to interaction of alpha-synuclein with lipid membranes. They are especially beneficial for sensing in scattering media such as solutions of lipid vesicles. We show that the described probes permit quantitative measurements of ot-synuclein binding to lipid membranes at low nanomolar concentrations. We developed a detailed protocol for measuring Kd and binding stoichiometry for interaction of soluble peripheral proteins with membranes based on the response of the environmentally sensitive fluorescent probes. We applied this protocol for quantification of the affinity of alpha-synuclein to anionic membranes and found that it is substantially higher than it was earlier reported.
    Trvalý link: http://hdl.handle.net/11104/0272145