Počet záznamů: 1  

Intrinsically disordered proteins drive enamel formation via an evolutionarily conserved self-assembly motif

  1. 1. 0474116 - MBU-M 2018 RIV US eng J - Článek v odborném periodiku
    Wald, Tomáš - Špoutil, František - Osičková, Adriana - Procházková, Michaela - Benada, Oldřich - Kašpárek, Petr - Bumba, Ladislav - Klein, O. D. - Sedláček, Radislav - Šebo, Peter - Procházka, Jan - Osička, Radim
    Intrinsically disordered proteins drive enamel formation via an evolutionarily conserved self-assembly motif.
    Proceedings of the National Academy of Sciences of the United States of America. Roč. 114, č. 9 (2017), s. 1641-1650 ISSN 0027-8424
    Grant CEP: GA MŠk(CZ) LM2015064; GA MŠk(CZ) LQ1604; GA MŠk(CZ) LM2011032; GA MŠk(CZ) LM2015040; GA MŠk(CZ) LO1509; GA MŠk(CZ) ED1.1.00/02.0109; GA MŠk ED2.1.00/19.0395
    Grant ostatní:Ministerstvo pro místní rozvoj(CZ) CZ2.16/3.1.00/24023
    Institucionální podpora: RVO:61388971 ; RVO:68378050
    Klíčová slova: ameloblastin * amelogenin * biomineralization
    Kód oboru RIV: EE - Mikrobiologie, virologie; EB - Genetika a molekulární biologie (UMG-J)
    Obor OECD: Microbiology; Microbiology (UMG-J)
    Impakt faktor: 9.504, rok: 2017

    The formation of mineralized tissues is governed by extracellular matrix proteins that assemble into a 3D organic matrix directing the deposition of hydroxyapatite. Although the formation of bones and dentin depends on the self-assembly of type I collagen via the Gly-X-Y motif, the molecular mechanism by which enamel matrix proteins (EMPs) assemble into the organic matrix remains poorly understood. Here we identified a Y/F-x-x-Y/L/F-x-Y/F motif, evolutionarily conserved from the first tetrapods to man, that is crucial for higher order structure self-assembly of the key intrinsically disordered EMPs, ameloblastin and amelogenin. Using targeted mutations in mice and high-resolution imaging, we show that impairment of ameloblastin self-assembly causes disorganization of the enamel organic matrix and yields enamel with disordered hydroxyapatite crystallites. These findings define a paradigm for the molecular mechanism by which the EMPs self-assemble into supramolecular structures and demonstrate that this process is crucial for organization of the organic matrix and formation of properly structured enamel.
    Trvalý link: http://hdl.handle.net/11104/0271237