Počet záznamů: 1  

TALE-directed local modulation of H3K9 methylation shapes exon recognition

  1. 1.
    0473083 - ÚMG 2017 RIV GB eng J - Článek v odborném periodiku
    Bieberstein, Nicole - Kozáková, Eva - Huranová, Martina - Thakur, P.K. - Krchňáková, Zuzana - Krausová, Michaela - Oesterreich, F.C. - Staněk, David
    TALE-directed local modulation of H3K9 methylation shapes exon recognition.
    Scientific Reports. Roč. 6, jaro (2016), č. článku 29961. ISSN 2045-2322. E-ISSN 2045-2322
    Grant CEP: GA ČR(CZ) GBP305/12/G034
    Institucionální podpora: RVO:68378050
    Klíčová slova: histone h3 * human genome * efficient design * chromatin * methyltransferase * transcription * trimethylation * identification * recruitment * annotation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.259, rok: 2016

    In search for the function of local chromatin environment on pre-mRNA processing we established a new tool, which allows for the modification of chromatin using a targeted approach. Using Transcription Activator-Like Effector domains fused to histone modifying enzymes (TALE-HME), we show locally restricted alteration of histone methylation modulates the splicing of target exons. We provide evidence that a local increase in H3K9 di- and trimethylation promotes inclusion of the target alternative exon, while demethylation by JMJD2D leads to exon skipping. We further demonstrate that H3K9me3 is localized on internal exons genome-wide suggesting a general role in splicing. Consistently, targeting of the H3K9 demethylase to a weak constitutive exon reduced co-transcriptional splicing. Together our data show H3K9 methylation within the gene body is a factor influencing recognition of both constitutive and alternative exons.
    Trvalý link: http://hdl.handle.net/11104/0270253

     
    Název souboruStaženoVelikostKomentářVerzePřístup
    Scientific_Reports_N_Bieberstein_2016.pdf1791.5 KBVydavatelský postprintvyžádat
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.