0472674 - ÚMCH 2018 RIV US eng J - Článek v odborném periodiku
Riedel, Tomáš - Hageneder, S. - Surman, František - Pop-Georgievski, Ognen - Noehammer, C. - Hofner, M. - Brynda, Eduard - Rodriguez-Emmenegger, Cesar - Dostálek, J.
Plasmonic hepatitis B biosensor for the analysis of clinical saliva.
Analytical Chemistry. Roč. 89, č. 5 (2017), s. 2972-2977. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA ČR(CZ) GJ15-09368Y; GA ČR(CZ) GBP205/12/G118; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Grant ostatní: OPPK(XE) CZ.2.16/3.1.00/21545
Program: OPPK
Institucionální podpora: RVO:61389013
Klíčová slova: surface plasmon-enhanced fluorescence spectroscopy * biosensors * polymer brushes
Obor OECD: Polymer science
Impakt faktor: 6.042, rok: 2017

A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.
Trvalý link: http://hdl.handle.net/11104/0271128