Počet záznamů: 1  

The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds-an in vitro evaluation using mesenchymal stem cells

  1. 1.
    0472325 - ÚSMH 2017 RIV GB eng J - Článek v odborném periodiku
    Suchý, Tomáš - Šupová, Monika - Sauerová, P. - Verdánová, M. - Sucharda, Zbyněk - Rýglová, Šárka - Žaloudková, Margit - Sedláček, R. - Hubálek Kalbáčová, M.
    The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds-an in vitro evaluation using mesenchymal stem cells.
    Biomedical Materials. Roč. 10, DEC (2015), č. článku 065008. ISSN 1748-6041. E-ISSN 1748-605X
    Grant CEP: GA MZd(CZ) NV15-25813A
    Institucionální podpora: RVO:67985891
    Klíčová slova: cross-linking agents * nano-composite scaffolds * human mesenchymal stem cells * EDC/NHS * genipin
    Kód oboru RIV: JI - Kompozitní materiály
    Impakt faktor: 3.361, rok: 2015

    Nanocomposite scaffolds which aimed to imitate a bone extracellular matrix were prepared for bone surgery applications. The scaffolds consisted of polylactide electrospun nano/sub-micron fibres, a natural collagen matrix supplemented with sodium hyaluronate and natural calcium phosphate nano-particles (bioapatite). The mechanical properties of the scaffolds were improved by means of three different cross-linking agents: N-(3-dimethylamino propyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide in an ethanol solution (EDC/NHS/EtOH), EDC/NHS in a phosphate buffer saline solution (EDC/NHS/PBS) and genipin. The effect of the various cross-linking conditions on the pore size, structure and mechanical properties of the scaffolds were subsequently studied. In addition, the mass loss, the swelling ratio and the pH of the scaffolds were determined following their immersion in a cell culture medium. Furthermore, the metabolic activity of human mesenchymal stem cells (hMSCs) cultivated in scaffold infusions for 2 and 7 days was assessed. Finally, studies were conducted of cell adhesion, proliferation and penetration into the scaffolds. With regard to the structural stability of the tested scaffolds, it was determined that EDC/NHS/PBS and genipin formed the most effectively cross-linked materials. Moreover, it was discovered that the genipin cross-linked scaffold also provided the best conditions for hMSC cultivation. In addition, the infusions from all the scaffolds were found to be non-cytotoxic. Thus, the genipin and EDC/NHS/PBS cross-linked scaffolds can be considered to be promising biomaterials for further in vivo testing and bone surgery applications.
    Trvalý link: http://hdl.handle.net/11104/0269665

     
     
Počet záznamů: 1  

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