Počet záznamů: 1  

Characterization of telomeres and telomerase from the single-celled eukaryote Giardia intestinalis

  1. 1.
    0471934 - BFÚ 2017 RIV NL eng J - Článek v odborném periodiku
    Uzlíková, M. - Fulnečková, Jana - Weisz, F. - Sýkorová, Eva - Nohýnková, E. - Tůmová, P.
    Characterization of telomeres and telomerase from the single-celled eukaryote Giardia intestinalis.
    Molecular and Biochemical Parasitology. Roč. 211, JAN 2017 (2017), s. 31-38. ISSN 0166-6851. E-ISSN 1872-9428
    Grant ostatní: Grantová agentura ČR - GA ČR(CZ) GAP305/12/1248
    Institucionální podpora: RVO:68081707
    Klíčová slova: trypanosoma-brucei * parasitic protozoa * 2 nuclei * lamblia * evolution * rna * organization
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 1.744, rok: 2017

    The ends of linear chromosomes, telomeres, are most commonly maintained by the enzyme telomerase. Our study presents the characteristics of telomeres and telomerase from the single-celled parasitic eukaryote Giardia intestinalis. Using fluorescence in situ hybridization, we localized telomeres during all stages of the trophozoite cell cycle and demonstrated differences in the observed number of telomeric foci, indicating telomere clustering. The length of Giardia telomeres was determined in different cell lines derived from WB clinical isolate using terminal restriction fragment analysis and ranged from 0.5 to 2.5 kb; moreover, a BAL-31 digestion experiment did not reveal any long interstitial telomeric sequences in the genome. Despite the absence of the specific T motif in the telomerase catalytic subunit, the presence of an active telomerase enzyme synthesising telomeric repeats in Giardia was proved by a Telomere repeat amplification protocol assay, and its localization in nuclei was determined by the expression of recombinant GiTERT. Except for the Giardia-type TAGGG telomeric repeat, Giardia telomerase was proved to synthesize in vitro also other repeat variants, TAAGG and TAAGGG. In summary, despite its unusual characteristics, including a structurally divergent but active telomerase, unique terminal sequences and relatively short telomeres, the present data support the view that the chromosomal termini in Giardia are maintained in a conservative manner that is common to other eukaryotes. (C) 2016 Elsevier B.V. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0269296

     
     
Počet záznamů: 1  

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