Počet záznamů: 1  

Distinct Immunoregulatory Mechanisms in Mesenchymal Stem Cells: Role of the Cytokine Environment

  1. 1.
    0470890 - ÚEM 2017 RIV US eng J - Článek v odborném periodiku
    Holáň, Vladimír - Heřmánková, Barbora - Boháčová, Pavla - Kössl, Jan - Chudíčková, Milada - Hájková, Michaela - Krulová, Magdaléna - Zajícová, Alena - Javorková, Eliška
    Distinct Immunoregulatory Mechanisms in Mesenchymal Stem Cells: Role of the Cytokine Environment.
    Stem Cell Reviews. Roč. 12, č. 6 (2016), s. 654-663. ISSN 1550-8943. E-ISSN 1558-6804
    Grant CEP: GA ČR(CZ) GA14-12580S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LO1508; GA MŠMT(CZ) LO1309
    Institucionální podpora: RVO:68378041
    Klíčová slova: mesenchymal stem cells * regulatory B cells * cytokine environment
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.967, rok: 2016

    Mesenchymal stem cells (MSCs) represent a population of cells which have the ability to regulate reactivity of T and B lymphocytes by multiple mechanisms. The immunoregulatory activities of MSCs are strictly influenced by the cytokine environment. Here we show that two functionally distinct cytokines, interleukin-4 (IL-4) and interferon-gamma (IFN-gamma), significantly potentiate the ability of MSCs to inhibit IL-10 production by activated regulatory B cells (Bregs). However, MSCs in the presence of IL-4 or IFN-gamma inhibit the IL-10 production by different mechanisms. Preincubation of MSCs with IFN-gamma led to the suppression, but pretreatment with IL-4 of neither MSCs nor B cells resulted in the suppression of IL-10 production. The search for candidate regulatory molecules expressed in cytokine-treated MSCs revealed different patterns of the gene expression. Pretreatment of MSCs with IFN-gamma, but not with IL-4, induced expression of indoleamine-2,3-dioxygenase, cyclooxygenase-2 and programmed cell death-ligand 1. To identify the molecule(s) responsible for the suppression of IL-10 production, we used specific inhibitors of the putative regulatory molecules. We found that indomethacine, an inhibitor of cyclooxygenase-2 (Cox-2) activity, completely abrogated the inhibition of IL-10 production in cultures containing MSCs and IFN-gamma, but had no effect on the suppression in cell cultures containing MSCs and IL-4. The results show that MSCs can inhibit the response of B cells to one stimulus by different mechanisms in dependence on the cytokine environment and thus support the idea of the complexity of immunoregulatory action of MSCs
    Trvalý link: http://hdl.handle.net/11104/0268400

     
     
Počet záznamů: 1  

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