Počet záznamů: 1  

Toxic Effects of the Major Components of Diesel Exhaust in Human Alveolar Basal Epithelial Cells (A549)

  1. 1.
    0469914 - ÚEM 2017 RIV CH eng J - Článek v odborném periodiku
    Rössner ml., Pavel - Strapáčová, S. - Štolcpartová, Jitka - Schmuczerová, Jana - Milcová, Alena - Neča, J. - Vlková, Veronika - Brzicová, Táňa - Machala, M. - Topinka, Jan
    Toxic Effects of the Major Components of Diesel Exhaust in Human Alveolar Basal Epithelial Cells (A549).
    International Journal of Molecular Sciences. Roč. 17, č. 9 (2016), s. 1393. E-ISSN 1422-0067
    Grant CEP: GA ČR(CZ) GA13-01438S; GA ČR(CZ) GA16-14631S; GA MŠMT(CZ) LO1508; GA MŠMT(CZ) LM2015073
    Institucionální podpora: RVO:68378041
    Klíčová slova: benzo[a]pyrene * 1-nitropyrene * 3-nitrobenzanthrone
    Kód oboru RIV: DN - Vliv životního prostředí na zdraví
    Impakt faktor: 3.226, rok: 2016

    We investigated the toxicity of benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP) and 3-nitrobenzanthrone (3-NBA) in A549 cells. Cells were treated for 4 h and 24 h with: B[a]P (0.1 and 1 M), 1-NP (1 and 10 M) and 3-NBA (0.5 and 5 M). Bulky DNA adducts, lipid peroxidation, DNA and protein oxidation and mRNA expression of CYP1A1, CYP1B1, NQO1, POR, AKR1C2 and COX2 were analyzed. Bulky DNA adducts were induced after both treatment periods; the effect of 1-NP was weak. 3-NBA induced high levels of bulky DNA adducts even after 4-h treatment, suggesting rapid metabolic activation. Oxidative DNA damage was not affected. 1-NP caused protein oxidation and weak induction of lipid peroxidation after 4-h incubation. 3-NBA induced lipid peroxidation after 24-h treatment. Unlike B[a]P, induction of the aryl hydrocarbon receptor, measured as mRNA expression levels of CYP1A1 and CYP1B1, was low after treatment with polycyclic aromatic hydrocarbon (PAH) nitro-derivatives. All test compounds induced mRNA expression of NQO1, POR, and AKR1C2 after 24-h treatment. AKR1C2 expression indicates involvement of processes associated with reactive oxygen species generation. This was supported further by COX2 expression induced by 24-h treatment with 1-NP. In summary, 3-NBA was the most potent genotoxicant, whereas 1-NP exhibited the strongest oxidative properties.
    Trvalý link: http://hdl.handle.net/11104/0267677

     
     
Počet záznamů: 1  

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