0469709 - ÚMCH 2017 RIV US eng J - Článek v odborném periodiku
Riabtseva, Anna - Kaberov, Leonid I. - Kučka, Jan - Bogomolova, Anna - Štěpánek, Petr - Filippov, Sergey K. - Hrubý, Martin
Polyelectrolyte pH-responsive protein-containing nanoparticles: the physicochemical supramolecular approach.
Langmuir : the ACS journal of surfaces and colloids. Roč. 33, č. 3 (2017), s. 764-772. ISSN 0743-7463. E-ISSN 1520-5827
Grant CEP: GA MŠMT(CZ) LH14292; GA MZd(CZ) NV15-25781A; GA ČR(CZ) GC15-10527J
Institucionální podpora: RVO:61389013
Klíčová slova: pH-sensitive polymers * Eudragit * nanocontainers
Obor OECD: Physical chemistry
Impakt faktor: 3.789, rok: 2017 ; AIS: 0.964, rok: 2017
DOI: https://doi.org/10.1021/acs.langmuir.6b03778

We report on the physicochemical properties and self-assembly behavior of novel efficient pH-sensitive nanocontainers based on the Food and Drug Administration-approved anionic polymer Eudragit L100-55 (poly(methacrylic acid-co-ethyl acrylate) 1:1) and nonionic surfactant Brij98. The features of the interaction between Eudragit L100-55 and Brij98 at different pH values and their optimal ratio for nanoparticle formation were studied using isothermal titration calorimetry. The influence of the polymer-to-surfactant ratio on the size and structure of particles was studied at different pH values using dynamic light scattering and small-angle X-ray scattering methods. It was shown that stable nanoparticles are formed at acidic pH at polymer-to-surfactant molar ratios from 1:43 to 1:139. Trypsin was successfully encapsulated into Eudragit-Brij98 nanoparticles as a model bioactive component. The loading efficiency was determined by labeling trypsin with radioactive iodine-125. Eudragit-Brij98 nanoparticles effectively protected trypsin against pepsin digestion. The results showed that trypsin encapsulated into novel pH-sensitive nanocontainers retained more than 50% of its activity after treatment with pepsin compared with nonencapsulated trypsin. The described concept will contribute both to understanding the principles of and designing next-generation nanocontainers.
Trvalý link: http://hdl.handle.net/11104/0269129