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Fraction transfer process in on-line comprehensive two-dimensional liquid phase separations

  1. 1.
    0469572 - ÚIACH 2017 RIV DE eng J - Článek v odborném periodiku
    Česla, P. - Křenková, Jana
    Fraction transfer process in on-line comprehensive two-dimensional liquid phase separations.
    Journal of Separation Science. Roč. 40, č. 1 (2017), s. 109-123. ISSN 1615-9306. E-ISSN 1615-9314
    Grant CEP: GA ČR(CZ) GA14-06319S
    Institucionální podpora: RVO:68081715
    Klíčová slova: capillary electrophoresis * comprehensive liquid chromatography * fraction transfer * two-dimensional separations * liquid chromatography
    Obor OECD: Analytical chemistry
    Impakt faktor: 2.415, rok: 2017

    Two-dimensional liquid-phase separations have gained increasing attention for their ability to separate complex sample mixtures. Among the experimental setups used, an on-line approach is preferred to reduce the probability of sample contamination, for easier automation and high-sample throughput. The interfacing of the separation techniques in the on-line mode brings additional demands on proper optimization of the two-dimensional system. In this review, the possibilities of the on-line coupling of liquid chromatography and liquid chromatography with capillary electrophoresis in two-dimensional systems are discussed. Special attention is paid to the fraction transfer process, which includes an overview of interfaces and experimental setups applied, the compatibility issues of separation systems, and instrumental parameters. The benefits and drawbacks of using electromigration separations in combination with liquid chromatography are presented as well.
    Trvalý link: http://hdl.handle.net/11104/0267406

     
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