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Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)

  1. 1.
    0468874 - MBÚ 2017 RIV US eng J - Článek v odborném periodiku
    Jerez, C.G. - Malapascua, José R. - Sergejevová, Magda - Figueroa, Felix L. - Masojídek, Jiří
    Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta).
    Marine Biotechnology. Roč. 18, č. 1 (2016), s. 24-36. ISSN 1436-2228. E-ISSN 1436-2236
    Grant CEP: GA MŠMT ED2.1.00/03.0110; GA MŠMT EE2.3.30.0059; GA MŠMT(CZ) EE2.3.20.0203
    Institucionální podpora: RVO:61388971
    Klíčová slova: Microalgae * Chlorella * Lipid
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.748, rok: 2016

    The effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved.
    Trvalý link: http://hdl.handle.net/11104/0267383

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