Počet záznamů: 1  

Proteome analysis of Bordetella pertussis isolated from human macrophages

  1. 1.
    0468787 - MBÚ 2017 RIV NL eng J - Článek v odborném periodiku
    Lamberti, Y. - Cafiero, J.H. - Surmann, K. - Valdez, H. - Holubová, Jana - Večerek, Branislav - Šebo, Peter - Schmidt, F. - Völker, U. - Rodriguez, M.E.
    Proteome analysis of Bordetella pertussis isolated from human macrophages.
    Journal of Proteomics. Roč. 136, MAY16 (2016), s. 55-67. ISSN 1874-3919. E-ISSN 1876-7737
    Grant CEP: GA MŠMT(CZ) 7AMB14AR028
    Institucionální podpora: RVO:61388971
    Klíčová slova: Bordetella pertussis * Intracellular survival * Proteomics
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.914, rok: 2016

    Previous studies have shown that B. pertussis survives inside human macrophages in non-acidic compartments with characteristics of early endosomes. In order to gain new insight into the biology of B. pertussis survival in host cells, we have analyzed the adaptation of the bacterial proteome during intracellular infection. The proteome of B. pertussis 3 h and 48 h after infection of human macrophage-like THP-1 cells was examined by nano-liquid chromatography combined with tandem MS and compared to the protein profile of extracellular B. pertussis growing in the same cell culture medium. Compared with extracellular bacteria, almost 300 proteins out of 762 identified proteins displayed altered levels in intracellular B. pertussis. Functional analyses of the proteins displaying altered abundance revealed enrichment of proteins involved in stress response, iron uptake, cellular metabolism, transcriptional regulation, and virulence.
    To our knowledge, this is the first analysis of the B. pertussis proteome during adaptation to the intramacrophage environment and the data provide new clues for understanding B. pertussis adaptation and pathogenesis.
    Biological significance: B. pertussis is a respiratory pathogen that has adapted exclusively to the human host. Despite high vaccination rates, whooping cough remains a serious threat to human health and its incidence has been increasing in recent years in vaccinated populations. The mechanisms that allow this pathogen to evade immune clearance, persist in the host, and cause a prolonged paroxysmal cough are still poorly understood. Recent studies regarding B. pertussis survival inside host cells and the cellular response to this bacterial infection indicate that B. pertussis may have an intracellular phase during infection which probably contributes to persistence and vaccine failure. In this study we provide the first global proteome profile of B. pertussis within macrophages.
    Trvalý link: http://hdl.handle.net/11104/0267386

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.