0468718 - BC 2017 RIV NL eng J - Článek v odborném periodiku
Pecková, R. - Stuart, P. D. - Sak, Bohumil - Květoňová, Dana - Kváč, Martin - Foitová, I.
Statistical comparison of excystation methods in Cryptosporidium parvum oocysts.
Veterinary Parasitology. Roč. 230, OCT 30 (2016), s. 1-5. ISSN 0304-4017. E-ISSN 1873-2550
Grant CEP: GA ČR(CZ) GAP505/11/1163
Institucionální podpora: RVO:60077344
Klíčová slova: Cryptosporidium parvum * excystation methods * in vitro cultivation * sodium hypochlorite * tlypsin
Kód oboru RIV: EG - Zoologie
Impakt faktor: 2.356, rok: 2016 ; AIS: 0.602, rok: 2016
DOI: https://doi.org/10.1016/j.vetpar.2016.10.007

Excystation of sporozoites of Cryptosporidium parvum from oocysts is essential for successful in vitro assays. It has also been traditionally used as a measure for oocyst viability and infectivity. Laboratories use various excystation protocols so there is a need to clarify which method is the best. In this study, six different protocols for in vitro excystation of C. parvum oocysts were compared to find the most efficient excystation method (expressed as percentage excystation). Tested protocols differed in chemical pre-incubation steps, excystation media or time of incubation.
There were significant differences in percentage of excysted oocysts among groups excysted by different methods. There were also significant differences in percentage of excysted oocysts between methods using pre-incubation with sodium hypochlorite and those without. The other variables examined; the presence of trypsin, kind of excystation medium and the incubation time, did not show statistical differences in percentage excystation among groups.
Pre-incubation steps which included sodium hypochlorite, enhancing the permeability of the oocysts were found to increase the excystation ratio and methods using this step were the most effective.
Trvalý link: http://hdl.handle.net/11104/0266549