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Strain of Synechocystis PCC 6803 with Aberrant Assembly of Photosystem NN Contains Tandem Duplication of a Large Chromosomal Region

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    0468455 - MBÚ 2017 RIV CH eng J - Článek v odborném periodiku
    Tichý, Martin - Bečková, Martina - Kopečná, Jana - Noda, J. - Sobotka, Roman - Komenda, Josef
    Strain of Synechocystis PCC 6803 with Aberrant Assembly of Photosystem NN Contains Tandem Duplication of a Large Chromosomal Region.
    Frontiers in Plant Science. Roč. 7, May 12 (2016), s. 648. ISSN 1664-462X. E-ISSN 1664-462X
    Grant CEP: GA MŠMT(CZ) LO1416
    Institucionální podpora: RVO:61388971
    Klíčová slova: Synechocystis 6803 * chlorophyll * photosystem I
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.291, rok: 2016

    Cyanobacterium Synechocysfis PCC 6803 represents a favored model organism for photosynthetic studies. Its easy transformability allowed construction of a vast number of Synechocystis mutants including many photosynthetically incompetent ones. However, it became clear that there is already a spectrum of Synechocystis "wild-type" substrains with apparently different phenotypes. Here, we analyzed organization of photosynthetic membrane complexes in a standard motile Pasteur collection strain termed PCC and two non-motile glucose-tolerant substrains (named here GT-P and GT-W) previously used as genetic backgrounds for construction of many photosynthetic site directed mutants. Although, both the GT-P and GT-W strains were derived from the same strain constructed and described by Williams in 1988, only GT-P was similar in pigmentation and in the compositions of Photosystem II (PSII) and Photosystem I (PSI) complexes to PCC. In contrast, GT-W contained much more carotenoids but significantly less chlorophyll (Chl), which was reflected by lower level of dimeric PSII and especially trimeric PSI. We found that GT-W was deficient in Chl biosynthesis and contained unusually high level of unassembled Dl-D2 reaction center, CP47 and especially CP43. Another specific feature of GT-W was a several fold increase in the level of the Ycf39-Hlip complex previously postulated to participate in the recycling of Chl molecules. Genome re-sequencing revealed that the phenotype of GT-W is related to the tandem duplication of a large region of the chromosome that contains 100 genes including ones encoding D1, Psb28, and other PSII-related proteins as well as Mg-protoporphyrin methylester cyclase (Cycl). Interestingly, the duplication was completely eliminated after keeping GT-W cells on agar plates under photoautotrophic conditions for several months.
    Trvalý link: http://hdl.handle.net/11104/0267393

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