0465861 - MBÚ 2017 RIV IE eng J - Článek v odborném periodiku
Pelantová, Helena - Bugáňová, Martina - Holubová, Martina - Šedivá, B. - Zemenová, Jana - Sýkora, D. - Kaválková, P. - Haluzík, M. - Železná, Blanka - Maletínská, Lenka - Kuneš, Jaroslav - Kuzma, Marek
Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination.
Molecular and Cellular Endocrinology. Roč. 431, C (2016), s. 88-100. ISSN 0303-7207. E-ISSN 1872-8057
Grant CEP: GA ČR GA13-14105S; GA MŠMT(CZ) LO1509
Institucionální podpora: RVO:61388971 ; RVO:61388963
Klíčová slova: NMR metabolomics * Mouse * Obesity
Kód oboru RIV: EE - Mikrobiologie, virologie; FB - Endokrinologie, diabetologie, metabolizmus, výživa (UOCHB-X)
Impakt faktor: 3.754, rok: 2016 ; AIS: 1.133, rok: 2016
DOI: https://doi.org/10.1016/j.mce.2016.05.003

Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-beta-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-beta-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation
Trvalý link: http://hdl.handle.net/11104/0265391