Počet záznamů: 1  

The Sustainable Release of Vancomycin and Its Degradation Products From Nanostructured Collagen/Hydroxyapatite Composite Layers

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    0465814 - ÚSMH 2017 RIV US eng J - Článek v odborném periodiku
    Suchý, Tomáš - Šupová, Monika - Klapková, E. - Horný, L. - Rýglová, Šárka - Žaloudková, Margit - Braun, Martin - Sucharda, Zbyněk - Ballay, R. - Veselý, J. - Chlup, H. - Denk, František
    The Sustainable Release of Vancomycin and Its Degradation Products From Nanostructured Collagen/Hydroxyapatite Composite Layers.
    Journal of Pharmaceutical Sciences. Roč. 105, č. 3 (2016), 1288-1294. ISSN 0022-3549. E-ISSN 1520-6017
    Grant CEP: GA TA ČR(CZ) TA04010330
    Institucionální podpora: RVO:67985891
    Klíčová slova: anti-infectives * HPLC * coating * controlled release * degradation products * drug delivery systems * nanoparticles * pharmacokinetics * polymeric drug delivery systems
    Kód oboru RIV: JI - Kompozitní materiály
    Impakt faktor: 2.713, rok: 2016

    Infections of the musculoskeletal system present a serious problem with regard to the field of orthopedic and trauma medicine. The aim of the experiment described in this study was to develop a resorbable nanostructured composite layer with the controlled elution of antibiotics. The layer is composed of collagen, hydroxyapatite nanoparticles, and vancomycin hydrochloride (10 wt%). The stability of the collagen was enhanced by means of cross-linking. Four cross-linking agents were studied, namely an ethanol solution, a phosphate buffer solution of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide, genipin, and nordihydroguaiaretic acid. High performance liquid chromatography was used so as to characterize the in vitro release rates of the vancomycin and its crystalline degradation antibiotically inactive products over a 21-day period. The maximum concentration of the released active form of vancomycin (approximately 265 mg/L) exceeded the minimum inhibitory concentration up to an order of 17 times without triggering the burst releasing effect. At the end of the experiment, the minimum inhibitory concentration was exceeded by up to 6 times (approximately 100 mg/L). It was determined that the modification of collagen with hydroxyapatite nanoparticles does not negatively influence the sustainable release of vancomycin. The balance of vancomycin and its degradation products was observed after 14 days of incubation. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0264556

     
     
Počet záznamů: 1  

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