0465585 - MBÚ 2017 RIV DE eng J - Článek v odborném periodiku
Pivodová, V. - Zahler, S. - Karas, D. - Valentová, Kateřina - Ulrichová, J.
In vitro study of 2,3-dehydrosilybin and its galloyl esters as potential inhibitors of angiogenesis.
Pharmazie. Roč. 71, č. 8 (2016), s. 478-483. ISSN 0031-7144. E-ISSN 0031-7144
Grant CEP: GA ČR(CZ) GA15-03037S
Institucionální podpora: RVO:61388971
Klíčová slova: NF-KAPPA-B * VASCULAR ENDOTHELIAL-CELLS * GROWTH-FACTOR
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.126, rok: 2016 ; AIS: 0.234, rok: 2016

2,3-Dehydrosilybin exhibits substantial anticancer and antiangiogenic effects, which can be potentially improved by semi-synthetic modification such as esterification with gallic acid. The aim of this study was to examine the potential antiangiogenic effect of 2,3-dehydrosilybin and its galloyl esters (3-O-galloyl-2,3-dehydrosilybin; 7-O-galloyl-2,3-dehydrosilybin; 20-O-galloyl-2,3-dehydrosilybin and 23-O-galloyl-2,3-dehydrosilybin) and to determine which molecular mechanism could be responsible for their activity. The effect on cell proliferation, tube formation, signal transduction pathways (PI3K/Akt and ERK) and the cell cycle was studied in human microvascular endothelial cells (HMEC). The results showed that all compounds decreased the growth of HMEC, but the strongest effect was observed for 20-O-galloyl-2,3-dehydrosilybin at 5 mu mol/l. In addition, at 5 and 10 mu mol/l, this was the only compound that significantly inhibited HMEC tube formation. Based on an assessment of Akt and ERK1/2 expression, we suggest that 20-O-galloyl-2,3-dehydrosilybin influences the angiogenic process through the Akt pathway.
Trvalý link: http://hdl.handle.net/11104/0264306