Počet záznamů: 1  

Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts

  1. 1.
    0462041 - MBU-M 2017 RIV US eng J - Článek v odborném periodiku
    Bumba, Ladislav - Mašín, Jiří - Macek, Pavel - Wald, Tomáš - Motlová, Lucia - Bíbová, Ilona - Klímová, Nela - Bednárová, Lucie - Veverka, Václav - Kachala, M. - Svergun, D. I. - Bařinka, Cyril - Šebo, Peter
    Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts.
    Molecular Cell. Roč. 62, č. 1 (2016), s. 47-62. ISSN 1097-2765
    Grant CEP: GA MŠk(CZ) LK11205; GA MŠk(CZ) LO1304; GA ČR(CZ) GA15-11851S; GA ČR GA13-14547S; GA ČR GAP302/12/0460; GA ČR(CZ) GAP207/11/0717; GA MŠk(CZ) ED1.1.00/02.0109
    Institucionální podpora: RVO:61388971 ; RVO:61388963 ; RVO:86652036
    Klíčová slova: ADENYLATE-CYCLASE TOXIN * GRAM-NEGATIVE BACTERIA * BORDETELLA-PERTUSSIS
    Kód oboru RIV: CE - Biochemie; EB - Genetika a molekulární biologie (BTO-N); CE - Biochemie (UOCHB-X)
    Impakt faktor: 14.714, rok: 2016

    Calcium-binding RTX proteins are equipped with C-terminal secretion signals and translocate from the Ca2+-depleted cytosol of Gram-negative bacteria directly into the Ca2+-rich external milieu, passing through the "channel-tunnel" ducts of type I secretion systems (T1SSs). Using Bordetella pertussis adenylate cyclase toxin, we solved the structure of an essential C-terminal assembly that caps the RTX domains of RTX family leukotoxins. This is shown to scaffold directional Ca2+-dependent folding of the carboxy-proximal RTX repeat blocks into beta-rolls. The resulting intramolecular Brownian ratchets then prevent backsliding of translocating RTX proteins in the T1SS conduits and thereby accelerate excretion of very large RTX leukotoxins from bacterial cells by a vectorial "push-ratchet" mechanism.
    Trvalý link: http://hdl.handle.net/11104/0261587