Počet záznamů: 1  

Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases

  1. 1. 0458981 - UOCHB-X 2017 RIV GB eng J - Článek v odborném periodiku
    Kielkowski, Pavel - Cahová, Hana - Pohl, Radek - Hocek, Michal
    Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
    Bioorganic & Medicinal Chemistry. Roč. 24, č. 6 (2016), s. 1268-1276. ISSN 0968-0896
    Grant CEP: GA ČR GBP206/12/G151
    Institucionální podpora: RVO:61388963
    Klíčová slova: nucleosides * nucleotides * pyrimidines * DNA methyltransferases * DNA polymerases
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 2.930, rok: 2016

    New types of double-headed 2'-deoxycytidine 5'-0-triphosphates (dOcTPs) bearing another cytosine or 5-fluorocytosine linked through a flexible propargyl, homopropargyl or pent-1-ynyl linker to position 5 were prepared by the aqueous Sonogashira cross -coupling reactions of 5-iodo-dCTP with the corresponding (fluoro)cytosine-alkynes. The modified dCxcTPs were good substrates for DNA polymerases and were used for enzymatic synthesis of cytosine-functionalized DNA by primer extension or PCR. The cytosine or fluorocytosine-linked DNA probes did not significantly inhibit DNA methyltransferases and did not cross-link to these proteins.
    Trvalý link: http://hdl.handle.net/11104/0259179