Počet záznamů: 1
DNA damage response during mouse oocyte maturation
- 1. 0458917 - UZFG-Y 2017 RIV US eng J - Článek v odborném periodiku
Mayer, Alexandra - Baran, Vladimír - Sakakibara, Y. - Brzáková, Adéla - Ferencová, Ivana - Motlík, Jan - Kitajima, T. - Schultz, R. M. - Šolc, Petr
DNA damage response during mouse oocyte maturation.
Cell Cycle. Roč. 15, č. 4 (2016), s. 546-558 ISSN 1538-4101
Grant CEP: GA MŠk LH12057; GA MŠk ED2.1.00/03.0124
Institucionální podpora: RVO:67985904
Klíčová slova: double strand DNA breaks * DNA damage * MRE11 * meiotic maturation * mouse oocytes
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.530, rok: 2016
Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels of DSBs induced by the radiomimetic drug Neocarzinostatin (NCS) increase the incidence of chromosome fragments and lagging chromosomes but do not lead to APC/C activation and anaphase onset delay. The number of DSBs, represented by gamma H2AX foci, significantly decreases between prophase I and metaphase II in both control and NCS-treated oocytes. Transient treatment with NCS increases >2-fold the number of DSBs in prophase I oocytes, but less than 30% of these oocytes enter anaphase with segregation errors. MRE11, but not ATM, is essential to detect DSBs in prophase I and is involved in H2AX phosphorylation during metaphase I. Inhibiting MRE11 by mirin during meiotic maturation results in anaphase bridges and also increases the number of gamma H2AX foci in metaphase II. Compromised DNA integrity in mirin-treated oocytes indicates a role for MRE11 in chromosome integrity during meiotic maturation.
Trvalý link: http://hdl.handle.net/11104/0259129