Počet záznamů: 1
Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis
- 1.0455588 - MBÚ 2016 RIV US eng J - Článek v odborném periodiku
Čáp, M. - Váchová, Libuše - Palková, Z.
Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis.
Cell Cycle. Roč. 14, č. 21 (2015), s. 3488-3497. ISSN 1538-4101. E-ISSN 1551-4005
Grant CEP: GA ČR(CZ) GA15-08225S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: enzymatic assays * fermentation * metabolic differentiation
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.952, rok: 2015
Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis.
Trvalý link: http://hdl.handle.net/11104/0256186
Počet záznamů: 1