Počet záznamů: 1  

Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization

  1. 1.
    0455579 - MBU-M 2016 RIV US eng J - Článek v odborném periodiku
    De Winter, K. - Dewitte, W. - Dirks-Hofmeister, M. E. - De Laet, S. - Pelantová, Helena - Křen, Vladimír - Desmet, T.
    Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization.
    Journal of Agricultural and Food Chemistry. Roč. 63, č. 46 (2015), s. 10131-10139. ISSN 0021-8561
    Grant CEP: GA MŠk(CZ) 7E11011; GA MŠk(CZ) LD13042
    Institucionální podpora: RVO:61388971
    Klíčová slova: glycosylation * antioxidant * ABTS
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.857, rok: 2015

    Although numerous biologically active molecules exist as glycosides in nature, information on the activity, stability, and solubility of glycosylated antioxidants is rather limited to date. In this work, a wide variety of antioxidants were glycosylated using different phosphorylase enzymes. The resulting antioxidant library, containing alpha/beta-glucosides, different regioisomers, cellobiosides, and cellotriosides, was then characterized. Glycosylation was found to significantly increase the solubility and stability of all evaluated compounds. Despite decreased radical-scavenging abilities, most glycosides were identified to be potent antioxidants, outperforming the commonly used 2,6-bis(1,1-dimethylethyl)-4-methylphenol (BHT). Moreover, the point of attachment, the anomeric configuration, and the glycosidic chain length were found to influence the properties of these phenolic glycosides.
    Trvalý link: http://hdl.handle.net/11104/0256210