Počet záznamů: 1  

Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes

  1. 1.
    0453597 - UFCH-W 2017 RIV AT eng J - Článek v odborném periodiku
    Navrátil, Tomáš - Nováková, Kateřina - Josypčuk, Bohdan - Sokolová, Romana - Šestáková, Ivana
    Voltammetric detection of phytochelatin transported across unmodified and protoplast modified model phospholipid membranes.
    Monatshefte fur Chemie. Roč. 147, č. 1 (2016), s. 165-171. ISSN 0026-9247
    Grant CEP: GA ČR(CZ) GAP208/12/1645
    Grant ostatní:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M200401201
    Program:M
    Institucionální podpora: RVO:61388955
    Klíčová slova: Barley protoplasts * electrochemical impendance spectroscopy * mercury electrode
    Kód oboru RIV: CG - Elektrochemie
    Impakt faktor: 1.282, rok: 2016

    Possibility of phytochelatin PC2 transport was
    examined using developed electrochemical cell with two
    compartments separated by a phospholipid membrane
    created in pores of polycarbonate support. At first cell
    penetrating peptides (transportan 10 and mastoparan X)
    were used to allow the transfer of phytochelatin PC2. The
    results demonstrated that transportan 10 is several times
    more efficient than mastoparan X for transferring of phytochelatin
    across model membrane. Voltammetry with
    hanging mercury drop electrode or with solid amalgam
    electrodes was applied for detection of transported species.
    For determination of PC2, hanging mercury drop electrode
    and mercury meniscus-modified silver solid amalgam
    electrode were suitable. Modified model phospholipid
    membrane was prepared with parts of protoplasts from
    barley roots or leaves, and transport of phytochelatin PC2
    has been confirmed. Electrochemical impedance spectroscopy
    was utilized to monitor formation, stability, and
    qualitatively the transporting process across the supported
    phospholipid membranes.
    Trvalý link: http://hdl.handle.net/11104/0254366
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