Raman spectroscopy for reproducible bacterial and yeast biofilm identification

0451049 - ÚPT 2016 DE eng A - Abstrakt
Samek, Ota - Bernatová, Silvie - Kaňka, Jan - Ježek, Jan - Pilát, Zdeněk - Krzyžánek, Vladislav - Šiler, Martin - Zemánek, Pavel - Mlynariková, K. - Růžička, F.
Raman spectroscopy for reproducible bacterial and yeast biofilm identification.
Invitation and Programm 116th Annual Meeting of the DGaO. Erlangen: DGaO, 2015. s. 24.
[Annual Meeting of the DGaO /116./. 26.05.2015-29.05.2015, Brno]
Grant CEP: GA ČR GAP205/11/1687; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: spektroskopie
Kód oboru RIV: BH - Optika, masery a lasery

We report on Raman spectroscopy measurements for the selected bacterial and yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic.
Trvalý link: http://hdl.handle.net/11104/0252254