Počet záznamů: 1  

Selenium supplementation induces mitochondrial biogenesis in trophoblasts

  1. 1. 0450924 - BTO-N 2016 RIV GB eng J - Článek v odborném periodiku
    Khera, A. - Dong, L. F. - Holland, O. - Vanderlelie, J. - Pasdar, E.A. - Neužil, Jiří - Perkins, A.V.
    Selenium supplementation induces mitochondrial biogenesis in trophoblasts.
    Placenta. Roč. 36, č. 8 (2015), s. 363-369. ISSN 0143-4004
    Institucionální podpora: RVO:86652036
    Klíčová slova: Selenium * Reactive oxygen species * Mitochondrial biogenesis
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.972, rok: 2015

    Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.
    Trvalý link: http://hdl.handle.net/11104/0252119