Low flow electrospray interfacing CE/MS

0448769 - ÚIACH 2016 FR eng A - Abstrakt
Foret, František - Týčová, Anna - Křenková, Jana - Klepárník, Karel
Low flow electrospray interfacing CE/MS.
ITP2015/NoSSS2015. Final program and book of abstracts. 2015. L-10. ISBN N.
[International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015) /22./ and the Nordic Separation Science (NoSSS) symposium /8./. 30.08.2015-03.09.2015, Helsinki]
Grant CEP: GA ČR(CZ) GBP206/12/G014
Institucionální podpora: RVO:68081715
Klíčová slova: electrospray * CE/MS * GAP20612G014
Kód oboru RIV: CB - Analytická chemie, separace

In this work we have explored liquid junction interfacing for standard (50-75 mm ID) separation capillaries as well as the “interface-free” approach, where the CE/MS analysis was performed in narrow bore (<20 mm ID) electrospray capillary. The separation capillary and electrospray tip formed one entity and the high voltage, applied at the injection end of the capillary served for both the separation and electrospray ionization. Thus the separation voltage was defined as the product of the electrospray current and resistivity of the separation electrolyte. Optimum conditions for the separation and electrospray ionization were achieved with voltage programming. The performance of this simplest possible CE/MS system was tested on peptide separations from the cytochrome c tryptic digest. The subnanoliter sample consumption and sensitivity in the attomole range predetermines such a system for analysis of limited samples.
Trvalý link: http://hdl.handle.net/11104/0250394