0447214 - ÚIACH 2016 RIV US eng J - Článek v odborném periodiku
Lenobel, R. - Řehulková, H. - Šebela, M. - Franc, V. - Kahle, Vladislav - Moravcová, Dana - Řehulka, P.
Analysis of peptide mixtures for proteomics research using LC–ESI-MS with a simple microgradient device.
Lc Gc North America. Roč. 33, č. 6 (2015), s. 420-428. ISSN 1527-5949. E-ISSN 1939-1889
Grant CEP: GA MV VG20112015021
Institucionální podpora: RVO:68081715
Klíčová slova: LC-ESI-MS * proteomics * peptide
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 0.519, rok: 2014

This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography and electrospray ionization tandem mass spectrometry. The system was successfully tested for a complex peptide mixture originating from the digestion of the whole-cell lysate from Francisella tularensis subsp. Schu S4 and it represents a cheap alternative for the separation of both simple and complex peptide mixtures prior to MS analysis.
Trvalý link: http://hdl.handle.net/11104/0249120