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Evalution of the diet of a carabid predator using fluorescent marking of prey

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    0446125 - BC 2016 RIV CZ eng J - Článek v odborném periodiku
    Okrouhlík, Jan - Foltan, Pavel
    Evalution of the diet of a carabid predator using fluorescent marking of prey.
    European Journal of Entomology. Roč. 112, č. 3 (2015), s. 477-485. ISSN 1210-5759. E-ISSN 1802-8829
    Grant ostatní: GA ČR(CZ) GA526/09/1249
    Institucionální podpora: RVO:60077344
    Klíčová slova: food web * prey marking * fluorescent dye
    Kód oboru RIV: EH - Ekologie - společenstva
    Impakt faktor: 0.954, rok: 2015
    http://www.eje.cz/pdfs/eje/2015/03/11.pdf

    We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey.
    Trvalý link: http://hdl.handle.net/11104/0250471

     
     
Počet záznamů: 1