HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

0441300 - BFÚ 2015 RIV GB eng J - Článek v odborném periodiku
Stixová, Lenka - Sehnalová, Petra - Legartová, Soňa - Suchánková, Jana - Hrušková, Tereza - Sorokin, D.V. - Matula, Pa. - Raška, I. - Kovařík, Aleš - Fulneček, Jaroslav - Bártová, Eva
HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs.
Epigenetics & Chromatin. Roč. 7, DEC2014 (2014), s. 1-17. E-ISSN 1756-8935
Grant CEP: GA ČR(CZ) GAP302/10/1022; GA ČR(CZ) GBP302/12/G157; GA ČR(CZ) GA13-07822S
Institucionální podpora: RVO:68081707
Klíčová slova: DNA-damage response * DNA repair * Irradiation
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 5.333, rok: 2014

Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.
Trvalý link: http://hdl.handle.net/11104/0244360