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Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low-Molecular-Weight Analytes

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    0441142 - ÚFE 2015 RIV DE eng J - Článek v odborném periodiku
    Sharma, N. - Petri, C. - Jonas, U. - Bach, M. - Tovar, G. - Mrkvová, Kateřina - Vala, Milan - Homola, Jiří - Knoll, W. - Dostálek, J.
    Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low-Molecular-Weight Analytes.
    Macromolecular Chemistry and Physics. Roč. 215, č. 23 (2014), s. 2295-2304. ISSN 1022-1352. E-ISSN 1521-3935
    Grant CEP: GA ČR GBP205/12/G118
    Institucionální podpora: RVO:67985882
    Klíčová slova: Label-free biosensors * Molecularly imprinted polymers * Hydrogels
    Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
    Impakt faktor: 2.616, rok: 2014

    New composite layer architecture of 3D hydrogel polymer network that is loaded with molecularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low-molecular-weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding-matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label-free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photocrosslinkable poly(N-isopropylacrylamide)-based hydrogel and poly[(ethylene glycol dimethylacrylate)-(methacrylic acid)] nanoparticles that are imprinted with L-Boc-phenylalanine-anilide (L-BFA, molecular weight 353 g mol(-1)). Titration experiments with the specific target and other structurally similar reference compounds show good specifi city and limit of detection for target L-BFA as low as 2 x 10(-6) M
    Trvalý link: http://hdl.handle.net/11104/0244193

     
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