Počet záznamů: 1
Cytokinin modulates proteomic, transcriptomic and growth responses to temperature shocks in Arabidopsis
- 1.0440595 - BFÚ 2015 RIV GB eng J - Článek v odborném periodiku
Černý, M. - Jedelsky, P. L. - Novák, Jan - Schlosser, A. - Brzobohatý, Břetislav
Cytokinin modulates proteomic, transcriptomic and growth responses to temperature shocks in Arabidopsis.
Plant Cell and Environment. Roč. 37, č. 7 (2014), s. 1641-1655. ISSN 0140-7791. E-ISSN 1365-3040
Institucionální podpora: RVO:68081707
Klíčová slova: Arabidopsis thaliana * cold * heat
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 6.960, rok: 2014
As sessile organisms, plants must sense environmental conditions and adjust their growth and development processes accordingly, through adaptive responses regulated by various internal factors, including hormones. A key environmental factor is temperature, but temperature-sensing mechanisms are not fully understood despite intense research. We investigated proteomic responses to temperature shocks (15min cold or heat treatments) with and without exogenous applications of cytokinin in Arabidopsis. Image and mass spectrometric analysis of the two-dimensionally separated proteins detected 139 differentially regulated spots, in which 148 proteins were identified, most of which have not been previously linked to temperature perception. More than 70% of the temperature-shock response proteins were modulated by cytokinin, mostly in a similar manner as heat shock. Data mining of previous transcriptomic datasets supported extensive interactions between temperature and cytokinin signalling. The biological significance of this finding was tested by assaying an independent growth response of Arabidopsis seedlings to heat stress: hypocotyl elongation.
Trvalý link: http://hdl.handle.net/11104/0243719
Počet záznamů: 1