Počet záznamů: 1  

Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve

  1. 1. 0440580 - BFU-R 2015 RIV GB eng J - Článek v odborném periodiku
    Dubový, P. - Brázda, Václav - Klusáková, I. - Hradilová-Svíženská, I.
    Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve.
    Journal of Neuroinflammation. Roč. 10, č. 55 (2013) E-ISSN 1742-2094
    Grant CEP: GA MŠk(CZ) ED1.1.00/02.0068
    Institucionální podpora: RVO:68081707
    Klíčová slova: Unilateral nerve injury * Contralateral reaction * Remote ganglia
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 4.902, rok: 2013

    Background: Current research implicates interleukin (IL)-6 as a key component of the nervous-system response to injury with various effects. Methods: We used unilateral chronic constriction injury (CCI) of rat sciatic nerve as a model for neuropathic pain. Immunofluorescence, ELISA, western blotting and in situ hybridization were used to investigate bilateral changes in IL-6 protein and mRNA in both lumbar (L4-L5) and cervical (C7-C8) dorsal root ganglia (DRG) following CCI. The operated (CCI) and sham-operated (sham) rats were assessed after 1, 3, 7, and 14 days. Withdrawal thresholds for mechanical hyperalgesia and latencies for thermal hyperalgesia were measured in both ipsilateral and contralateral hind and fore paws.Results: The ipsilateral hind paws of all CCI rats displayed a decreased threshold of mechanical hyperalgesia and withdrawal latency of thermal hyperalgesia, while the contralateral hind and fore paws of both sides exhibited no significant changes in mechanical or thermal sensitivity. No significant behavioral changes were found in the hind and fore paws on either side of the sham rats, except for thermal hypersensitivity, which was present bilaterally at 3 days.
    Trvalý link: http://hdl.handle.net/11104/0243708