Počet záznamů: 1  

Direct chemical modification and voltammetric detection of glycans in glycoproteins

  1. 1.
    0440098 - BFÚ 2015 RIV NL eng J - Článek v odborném periodiku
    Trefulka, Mojmír - Paleček, Emil
    Direct chemical modification and voltammetric detection of glycans in glycoproteins.
    Electrochemistry Communications. Roč. 48, NOV2014 (2014), s. 52-55. ISSN 1388-2481. E-ISSN 1873-1902
    Grant CEP: GA ČR(CZ) GAP301/11/2055
    Institucionální podpora: RVO:68081707
    Klíčová slova: Glycoproteins * Chemical modification * Os(VI)L complexes
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 4.847, rok: 2014

    Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0243234

     
     
Počet záznamů: 1  

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