0440098 - BFÚ 2015 RIV NL eng J - Článek v odborném periodiku
Trefulka, Mojmír - Paleček, Emil
Direct chemical modification and voltammetric detection of glycans in glycoproteins.
Electrochemistry Communications. Roč. 48, NOV2014 (2014), s. 52-55. ISSN 1388-2481. E-ISSN 1873-1902
Grant CEP: GA ČR(CZ) GAP301/11/2055
Institucionální podpora: RVO:68081707
Klíčová slova: Glycoproteins * Chemical modification * Os(VI)L complexes
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.847, rok: 2014 ; AIS: 1.162, rok: 2014
DOI: https://doi.org/10.1016/j.elecom.2014.08.011

Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0243234