Počet záznamů: 1  

Substitution-Inert Trinuclear Platinum Complexes Efficiently Condense/Aggregate Nucleic Acids and Inhibit Enzymatic Activity

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    0440095 - BFÚ 2015 RIV DE eng J - Článek v odborném periodiku
    Malina, Jaroslav - Farrell, N. P. - Brabec, Viktor
    Substitution-Inert Trinuclear Platinum Complexes Efficiently Condense/Aggregate Nucleic Acids and Inhibit Enzymatic Activity.
    Angewandte Chemie - International Edition. Roč. 53, č. 47 (2014), s. 12812-12816. ISSN 1433-7851. E-ISSN 1521-3773
    Grant CEP: GA ČR(CZ) GA13-08273S
    Institucionální podpora: RVO:68081707
    Klíčová slova: DNA condensation * nucleic acids * platinum
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 11.261, rok: 2014

    The trinuclear platinum complexes (Triplatin NC-A [{Pt(NH3)(3)}(2)-mu-{trans-Pt(NH3)(2)(NH2(CH2)(6)NH2)(2)}](6+), and Triplatin NC [{trans-Pt(NH3)(2)(NH2(CH2)(6)NH3+)}(2)-mu-{trans-Pt(NH3)(2)(NH2(CH2)(6)NH2)(2)}](8+)) are biologically active agents that bind to DNA through noncovalent (hydrogen bonding, electrostatic) interactions. Herein, we show that TriplatinNC condenses DNAwith a much higher potency than conventional DNA condensing agents. Both complexes induce aggregation of small transfer RNA molecules, and TriplatinNC in particular completely inhibits DNA transcription at lower concentrations than naturally occurring spermine. Topoisomerase Imediated relaxation of supercoiled DNA was inhibited by TriplatinNC-A and TriplatinNC at concentrations which were 60 times and 250 times lower than that of spermine. The mechanisms for the biological activity of TriplatinNC-A and TriplatinNC may be associated with their ability to condense/aggregate nucleic acids with consequent inhibitory effects on crucial enzymatic activities.
    Trvalý link: http://hdl.handle.net/11104/0243221

     
     
Počet záznamů: 1  

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