Počet záznamů: 1  

Synthesis of Base-Modified 2 '-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology

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    0438214 - ÚOCHB 2015 RIV US eng J - Článek v odborném periodiku
    Hocek, Michal
    Synthesis of Base-Modified 2 '-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology.
    Journal of Organic Chemistry. Roč. 79, č. 21 (2014), s. 9914-9921. ISSN 0022-3263. E-ISSN 1520-6904
    Grant CEP: GA ČR GBP206/12/G151; GA ČR GA14-04289S
    Institucionální podpora: RVO:61388963
    Klíčová slova: cross-coupling reactions * modified nucleoside triphosphates * nucleic acids
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 4.721, rok: 2014

    The synthesis of 2'-deoxyribonucleoside triphosphates (dNTPs) either by classical triphosphorylation of nucleosides or by aqueous cross-coupling reactions of halogenated dNTPs is discussed. Different enzymatic methods for synthesis of modified oligonucleotides and DNA by polymerase incorporation of modified nucleotides are summarized, and the applications in redox or fluorescent labeling, as well as in bioconjugations and modulation of interactions of DNA with proteins, are outlined.
    Trvalý link: http://hdl.handle.net/11104/0241677

     
     
Počet záznamů: 1  

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