0438214 - ÚOCHB 2015 RIV US eng J - Článek v odborném periodiku
Hocek, Michal
Synthesis of Base-Modified 2 '-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology.
Journal of Organic Chemistry. Roč. 79, č. 21 (2014), s. 9914-9921. ISSN 0022-3263. E-ISSN 1520-6904
Grant CEP: GA ČR GBP206/12/G151; GA ČR GA14-04289S
Institucionální podpora: RVO:61388963
Klíčová slova: cross-coupling reactions * modified nucleoside triphosphates * nucleic acids
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.721, rok: 2014 ; AIS: 1.035, rok: 2014
DOI: https://doi.org/10.1021/jo5020799

The synthesis of 2'-deoxyribonucleoside triphosphates (dNTPs) either by classical triphosphorylation of nucleosides or by aqueous cross-coupling reactions of halogenated dNTPs is discussed. Different enzymatic methods for synthesis of modified oligonucleotides and DNA by polymerase incorporation of modified nucleotides are summarized, and the applications in redox or fluorescent labeling, as well as in bioconjugations and modulation of interactions of DNA with proteins, are outlined.
Trvalý link: http://hdl.handle.net/11104/0241677