Počet záznamů: 1  

Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry

  1. 1.
    0437817 - FGÚ 2015 RIV NL eng J - Článek v odborném periodiku
    Pecina, Petr - Houšťková, H. - Mráček, Tomáš - Pecinová, Alena - Nůsková, Hana - Tesařová, M. - Hansíková, H. - Janota, J. - Zeman, J. - Houštěk, Josef
    Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry.
    Biochimica Et Biophysica Acta. Clinical. Roč. 2, Dec (2014), s. 62-71. ISSN 2214-6474
    Grant CEP: GA MZd(CZ) NT12370; GA ČR(CZ) GAP303/11/0970; GA ČR(CZ) GB14-36804G
    Institucionální podpora: RVO:67985823
    Klíčová slova: lymphocytes * respirometry * oxidative phosphorylation * mitochondrial diseases * diagnostics
    Kód oboru RIV: FG - Pediatrie

    To investigate manifestation of oxidative phosphorylation defects in isolated lymphocytes, digitonin-permeabilized cells from 48 children were analyzed by high resolution respirometry, cytofluorometric detection of mitochondrial membrane potential and immunodetection of respiratory chain proteins with SDS and Blue-Native electrophoreses. Based on our results, we propose the best biochemical parameters predictive for defects of respiratory complexes I, IV and V manifesting in peripheral blood lymphocytes. The noninvasiveness, reliability and speed of an approach utilizing novel biochemical criteria demonstrate the high potential of isolated lymphocytes for diagnostics of oxidative phosphorylation disorders in pediatric patients
    Trvalý link: http://hdl.handle.net/11104/0241349

     
     
Počet záznamů: 1  

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