Počet záznamů: 1  

L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration

  1. 1.
    0436786 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
    Serrano-Pertierra, E. - Cernuda-Morollón, E. - Brdička, Tomáš - Hořejší, Václav - López-Larrea, C.
    L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.
    Journal of Leukocyte Biology. Roč. 96, č. 3 (2014), s. 437-45. ISSN 0741-5400. E-ISSN 1938-3673
    Grant CEP: GA ČR(CZ) GBP302/12/G101
    Grant ostatní: Spanish Fondo de Investigaciones Sanitarias (FIS)(ES) PI12/02587; FICYT (Asturias, Spain)(ES) /
    Klíčová slova: chemotaxis * membrane rafts
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.289, rok: 2014

    Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by β-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstrated that L-plastin participates in NKG2D-mediated inhibition of NK cell chemotaxis.
    Trvalý link: http://hdl.handle.net/11104/0241961

     
     
Počet záznamů: 1  

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