0436781 - ÚMCH 2015 RIV CH eng J - Článek v odborném periodiku
Kučerová, J. - Svobodová, Z. - Knotek, P. - Palarčik, J. - Vlček, Milan - Kincl, Miloslav - Horák, Daniel - Autebert, J. - Viovy, J.-L. - Bílková, Z.
PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 40, 1 July (2014), s. 308-315. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA MŠMT 7E12053; GA MŠMT 7E09109
GRANT EU: European Commission(XE) 246513 - NADINE; European Commission(XE) 228980 - CAMINEMS
Institucionální podpora: RVO:61389013
Klíčová slova: PEGylation * magnetic microscopy * microfluidics
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.088, rok: 2014

Magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly(ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells. Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, and bioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres.
Trvalý link: http://hdl.handle.net/11104/0240475