0436649 - ÚŽFG 2015 RIV CZ eng J - Článek v odborném periodiku
Křivánek, J. - Švandová, Eva - Králik, J. - Hajda, S. - Fedr, Radek - Vinarsky, V. - Jaroš, J. - Souček, Karel - Buchtová, Marcela - Matalová, Eva - Hampl, Aleš
Properties of Neural Crest-Like Cells Differentiated from Human Embryonic Stem Cells.
Folia Biologica. Roč. 60, Suppl 1 (2014), s. 30-38. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA ČR GAP304/11/1418
Institucionální podpora: RVO:67985904 ; RVO:68081707
Klíčová slova: stem cell differentiation * neural crest * odontogenesis * cell therapies
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 1.000, rok: 2014 ; AIS: 0.238, rok: 2014

Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCC-like cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain heterogeneity of CD271 expression, and we showed that NCCs derived here possess the capacity to react to BMP4 signals by dramatically up-regulating MSX1, which is linked to odontogenesis.
Trvalý link: http://hdl.handle.net/11104/0240387