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Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

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    0436223 - ÚACH 2015 RIV GB eng J - Článek v odborném periodiku
    Rohanová, D. - Boccaccini, A. R. - Horkavcová, D. - Bozděchová, P. - Bezdička, Petr - Castoralová, M.
    Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?
    Journal of Materials Chemistry B. Roč. 2, č. 31 (2014), s. 5068-5076. ISSN 2050-750X. E-ISSN 2050-7518
    Institucionální podpora: RVO:61388980
    Klíčová slova: SIMULATED BODY-FLUID * HYDROXYAPATITE FORMATION
    Kód oboru RIV: CA - Anorganická chemie
    Impakt faktor: 4.726, rok: 2014

    Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solution failed to prevent formation of crystalline phases. The present results indicate that it is not recommendable to use DMEM for bioactivity tests of glass-ceramic materials due to its low concentration of Ca2+ ions, high concentration of HCO3- ions and the necessity to maintain sterile environment during the test.
    Trvalý link: http://hdl.handle.net/11104/0240001

     
     
Počet záznamů: 1  

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