0436065 - MBÚ 2015 RIV US eng J - Článek v odborném periodiku
Kostovčík, Martin - Bateman, C.C. - Kolařík, Miroslav - Stelinski, L.L. - Jordal, B.H. - Hulcr, J.
The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing.
The ISME Journal. Roč. 9, č. 1 (2015), s. 126-138. ISSN 1751-7362. E-ISSN 1751-7370
Institucionální podpora: RVO:61388971
Klíčová slova: ambrosia symbiosis * pyrosequencing
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 9.328, rok: 2015 ; AIS: 3.838, rok: 2015
DOI: https://doi.org/10.1038/ismej.2014.115

Symbioses are increasingly seen as dynamic ecosystems with multiple associates and varying fidelity. Symbiont specificity remains elusive in one of the most ecologically successful and economically damaging eukaryotic symbioses: the ambrosia symbiosis of wood-boring beetles and fungi. We used multiplexed pyrosequencing of amplified internal transcribed spacer II (ITS2) ribosomal DNA (rDNA) libraries to document the communities of fungal associates and symbionts inside the mycangia (fungus transfer organ) of three ambrosia beetle species, Xyleborus affinis, Xyleborus ferrugineus and Xylosandrus crassiusculus. We processed 93 beetle samples from 5 locations across Florida, including reference communities. Fungal communities within mycangia included 14–20 fungus species, many more than reported by culture-based studies.
Trvalý link: http://hdl.handle.net/11104/0239919