Počet záznamů: 1  

Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

  1. 1.
    0435787 - MBÚ 2015 RIV NL eng J - Článek v odborném periodiku
    Krásný, Lukáš - Rohlová, E. - Růžičková, E. - Šantrůček, J. - Hynek, R. - Hochel, I.
    Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis.
    Journal of Microbiological Methods. Roč. 98, MAR 2014 (2014), s. 105-113. ISSN 0167-7012. E-ISSN 1872-8359
    Grant CEP: GA ČR GAP503/10/0664
    Institucionální podpora: RVO:61388971
    Klíčová slova: Biotyper * Cronobacter * MALDI-TOF
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.026, rok: 2014

    Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species
    Trvalý link: http://hdl.handle.net/11104/0239660

     
     
Počet záznamů: 1  

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